The Potential Mechanism of Zishen Yutai Pills against Threatened Abortion: An Approach Involving Network Pharmacology and Experimental Evidence

Threatened abortion (TA) is the most common complication in early pregnancy and caused by anxiety depressive symptoms. The Zishen Yutai Pill (ZYP) a traditional herbal formula that commonly used to treat TA. However, pharmacological mechanisms underlying effect of ZYP have yet be elucidated. To disclose mechanism treatment TA, first, we identified chemical constituents from multiple databases then predicted potential targets TA applying GeneCards database. A protein-protein interaction (PPI) network was constructed allow screening hub targets. Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGGs) enrichment analyses were also performed Database for Annotation Visualization Integrated Discovery server identify critical biological processes signaling pathways. Cytoscape software construct Compound-Target-Pathway. Furthermore, analyzed UPLC-Q-TOF/MS, RU486-induced rat model established, reliability pharmacology prediction results verified. Finally, responsible action on revealed qRT-PCR molecular docking. total 161 active compounds 324 TA-related And, 42 UPLC-Q-TOF/MS. Inflammation apoptosis as main processes. GO KEGG MAPK PI3K/Akt pathways key functional respond ZYP. showed significantly increased maternal weight, levels estradiol progesterone, attenuated histopathological changes Data indicated improved outcomes QRT-PCR data reduced inflammation regulating In addition, molecule docking range compounds, including Pik3a, Mapk14, Mapk1, Mapk3, Mapk8 Tnf, Il6, Cas8. summary, analysis experimental validation exerts an inhibiting expression proinflammatory cytokines genes related apoptosis.